Metadata language
Wykrywanie pryszczycy z zastosowaniem PCR
Subtitle:Wykrywanie pryszczycy z zastosowaniem PCR
Creator:Paprocka, Grażyna ; Kęsy, Andrzej ; Fitzner, Andrzej ; Niedbalski, Wiesław ; Płucienniczak, Grażyna
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:The polymerase chain reaction (PCR) method has been applied to the detection of FMD viralRNA in samples taken during the clinical stage of FMD from calf. Total RNA was extracted withguanidinum thiocyanate-phenol-chloroform method and reversely transcribed using AMV-reversetranscriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of theVPl coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS(+). Thefirst DNA strand was sequenced and consistently an amino acid sequence was established.Comparison between VPl amino acid sequence of FMDV types C and earlier described type Owas performed.
Relation:Biotechnologia, vol.39, 4 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
Projects co-financed by: Access: