Inny tytuł:

Wykrywanie pryszczycy z zastosowaniem PCR

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

The polymerase chain reaction (PCR) method has been applied to the detection of FMD viralRNA in samples taken during the clinical stage of FMD from calf. Total RNA was extracted withguanidinum thiocyanate-phenol-chloroform method and reversely transcribed using AMV-reversetranscriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of theVPl coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS(+). Thefirst DNA strand was sequenced and consistently an amino acid sequence was established.Comparison between VPl amino acid sequence of FMDV types C and earlier described type Owas performed.

Czasopismo/Seria/cykl:

Biotechnologia, vol.39, 4 (1997)-.

Tom:

39

Zeszyt:

4

Strona pocz.:

55

Strona końc.:

61

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:145340 ; IChB B-35

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open