Inny tytuł:

Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD-PCR technique

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except ‘Węgierka Zwykła’ and clones ‘Promis’, ‘Tolar’, ‘Nectavit’.

Czasopismo/Seria/cykl:

Biotechnologia, vol.65, 2 (2004)-.

Tom:

65

Zeszyt:

2

Strona pocz.:

67

Strona końc.:

72

Szczegółowy typ zasobu:

Article

Format:

application/pdf ; application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:122353

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open