Nabiałkowska, Danuta ; Szarejko, Iwona ; Siwińska, Dorota
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Using the regeneration method described by Feldman and Marks (1986), the level of somaclonal variation (frequency of gene mutations and chromosomal changes) was determined in A. thaliana regenerants from root and shoot explants. The explants were regenerated via indirect organogenesis with callus stage. The level of embryo lethal mutations and changes in chromosome number were estimated in R2 generative progeny of regenerants, and confirmed in R3 generation. The frequency of R] plants carrying gene mutations was similar for all types of explants and on average it reached 8.3%, however only 47% of Ri plants showed the presence of the same embryo mutation in R2 and R3 generation. The frequency of embryo-lethal mutants, among 25 571 embryos tested, was 1.7%, while 0.08% embryos carried chlorophyll mutations. The plants with increased chromosome number were observed in the progeny of 32.2 % Rj regenerants, i.e. with eight times higher frequency than regenerants carrying gene mutations. All polyploid plants were obtained from shoot explants, while regenerants from roots carried normal diploid number of chromosomes. The observed changes in chromosome number were discussed in relation to the different content of DNA in root and shoot cells of A. thaliana.
Biotechnologia, vol.65, 2 (2004)-.
application/pdf ; application/pdf
0860-7796 ; oai:rcin.org.pl:122357
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Apr 29, 2020
335
https://rcin.org.pl/ichb/publication/152726
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia
Broniarczyk, Justyna Koczorowska, Maria Magdalena Durzyńska, Julia Warowicka, Alicja Goździcka-Józefiak, Anna