Micropropagation of Salvia officinalis L. by shoot tips
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 pmol/1 lAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 pmol/1 was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was 1/2 MS agar medium without growth regulators.
Biotechnologia, vol.65, 2 (2004)-.
application/pdf ; application/pdf
0860-7796 ; oai:rcin.org.pl:122751
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Apr 30, 2020
467
https://rcin.org.pl/ichb/publication/153182
Edition name | Date |
---|---|
Mikrorozmnażanie Salvia officinalis L. z wierzchołków pędów | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Wysokińska, Halina