Short interfering RNAs as tools for sequence-specific gene silencing
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
In diverse eukaryotes, dsRNA triggers the destruction of mRNA sharing thesame sequence as the dsRNA in the process called RNAi. The guides for sequence-specific degradation of mRNA are 21 nt short interfering RNAs (siRNAs).Synthetic siRNAs can efficiently mediate RNAi, but a drawback of RNAi is itstransient nature as a result of the limited availability and stability of syntheticoligonucleotides. Recently, several groups reported the construction of expression plasmid vectors that mediate the production of siRNAs under control ofPol III promoters. These vectors allow the continued expression of siRNAs in thecells resulting in persistent and specific suppression of target genes. The retroviralsiRNA expressing system allows for stable inactivation of the genes in primarycells or living organisms.
Biotechnologia, vol.61, 2 (2003)-.
0860-7796 ; oai:rcin.org.pl:135618 ; IChB B-57
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
2 paź 2020
19 sie 2020
417
https://rcin.org.pl/ichb/publication/170567
Nazwa wydania | Data |
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Krótkie interferujące RNA jako narzędzia do sekwencyjno-specyficznego wyciszania ekspresji genów | 2 paź 2020 |
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