Inny tytuł:

Propagation of Derzsy disease virus in goose embryo fibroblasts. An application of the Celligen Plus bioreactor

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Derzsy desease is a fatal hepatitis ofyoung geese under the age of 5 weeks.The etiological agent is a goose parvovirus (GPV) which is widespread in all major goose farming countries in Europe and Asia. At present, vaccination is theonly method preventing the loss of animals. In vitro GPV replicates only in gooseembryo fibroblasts (GEF) and this way the virus is propagated in order to obtainthe material for diagnostic tests and vaccine production.In this paper, we report the application of bioreactor Celligen Plus for GEFculture and Derzsy disease virus propagation. The method is based on immobilization and culture of cells on porous glass carriers in bioreactor. Comparing tothe traditional cell culture techniques, the described method was over 12-foldmore efficient and allowed to obtain good quality virus pool for Derzsy diseasevaccine preparation.

Czasopismo/Seria/cykl:

Biotechnologia, vol.61, 2 (2003)-.

Tom:

61

Zeszyt:

2

Strona pocz.:

261

Strona końc.:

267

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:135753 ; IChB B-57

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open