B-galactosidase activity of proteins extracted from Sulfolobus shibatae
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
The cytoplasmic p-galactosidase/p-glucosidase from hyperthermophilicarchaeon Sulfolobus shibatae has been characterized with regard to its use in lactose hydrolysis. Cell extract was purified 16-fold to a specific activity 29.5 U/mgusing ammonium sulfate precipitation, ion-exchange chromatography, and gelfiltration. Isolated enzyme exhibited optimum activity at pH 5.5 and 98°C andhad a half-life of 7 h in acetate buffer (pH 5.5) at 90°C. Cu2+, Hg2+ and Zn^+strongly inhibited the enzyme, whereas catalytic properties of other investigated cations were barely influenced. Glucose and galactose were predominantly produced from lactose. However, at the substrate concentration of 0.15 M,small amount of lactose was converted into transgalactosylation products.
Biotechnologia, vol.61, 2 (2003)-.
0860-7796 ; oai:rcin.org.pl:135754 ; IChB B-57
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
2 paź 2020
20 sie 2020
161
https://rcin.org.pl/ichb/publication/170596
Nazwa wydania | Data |
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Charakterystyka preparatów B-galaktozydazy z Sulfolobus shibatae | 2 paź 2020 |
Synowiecki, Józef Wołosowska, Sylwia
Wołosowska, Sylwia Synowiecki, Józef
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Grubiak, Katarzyna
Synowiecki, Józef
Nowak, Jacek K.