Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Research of the past decade has demonstrated that the use of enzymes andwhole-cell biocatalysts is environmentally friendly, economical and surprisingly,faces few barriers when applied in organic syntheses. In nature, enzymesevolved to function within a living system and may not exhibit features desirable for large-scale in vitro syntheses. Thus, protein engineering has the potential to dramatically enhance enzyme performance in a wide variety of unusual -but technologically important - environments. Site-directed, cassette-mutagenesis and construction of chimeric enzymes commonly used in rational protein engineering are important strategies which reveal the structure-functionrelationship of a given enzyme. Notably, construction of hybrid enzymes has become increasingly important in the rational design of novel biocatalysts withdesired properties and activities. However, “irrational” protein design based onrandom mutagenesis technologies or the combination with directed evolutionapproaches has truly revolutionized the generation offunctional biological molecules.
Biotechnologia, vol.58, 3 (2002)-.
0860-7796 ; oai:rcin.org.pl:137870 ; IChB B-54
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
2 paź 2020
4 wrz 2020
38
https://rcin.org.pl/ichb/publication/172999
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.