Golińska, Barbara ; Narożna, Dorota ; Mądrzak, Cezary J.
Molecular Methods for Detection, Identification and Characterization of Fusarium
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Pathogenic fungi of the genus Fusarium form a diverse, cosmopolitan groupofspecies. Their importance is significant due to the fact that they are responsible for numerous storage rots, plant diseases and human and animal diseasecaused by Fusarium toxic secondary metabolites. By reason of great extent ofvariability and plasticity of Fusarium easily accomodating to changing environmental condition, this genus comprises ofspecies that remain very challengingfor systematics. Since the reliable and simple detection methods are essentialto avoid the losses and health risks, molecular biology methos have been recently implemented for the analysis of Fusarium populations. In this paper, wepresent a review ofselected PCR-based techniques applicable for diagnostic andtaxonomic purposes. The RAPD analysis, followed by SCAR-PCR and RFLP methodto study fungal rDNA are presented. A number of primer sequences useful forthe above methods are also listed.
Biotechnologia, vol.58, 3 (2002)-.
0860-7796 ; oai:rcin.org.pl:137919 ; IChB B-54
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
2 paź 2020
4 wrz 2020
950
https://rcin.org.pl/ichb/publication/173029
Nazwa wydania | Data |
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Zastosowanie metod molekularnych do wykrywania, identyfikacjii charakterystyki grzybów z rodzaju Fusarium | 2 paź 2020 |
Narożna, Dorota Mądrzak, Cezary J.
Golińska, Barbara Mądrzak, Cezary J.
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.