Kinetic study of enzymatic hydrolysis of tannins
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Tannins are present in many plant foods. Tannase (EC 3.1.1.20) catalyses hydrolysis of ester bonds in hydrolysable gallotannins releasing glucose and gallicacid. Enzyme was produced by Penicillium chrysogenum cultured in Czapek-Doxmedium containing tannic acid. The tannic acid was used as a substrate in theenzymatic reactions. The product inhibits the enzymatic reaction. It was determined that inhibition was of the competitive type. The mathematical model(Eg. 7) and kinetic constancy describing the process were determined:Km = 0,53-10 ‘'|mol/dm3), Rm = 1,2610-3 (mol/dm^minj, Kp=2,04-10-'* (mol/dm^j.The results obtained from the model well agree with those obtained experimentally.
Biotechnologia, vol.57, 2 (2002)-.
0860-7796 ; oai:rcin.org.pl:138091 ; IChB B-53
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 7, 2020
218
https://rcin.org.pl/ichb/publication/173289
Edition name | Date |
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Badanie kinetyki enzymatycznej hydrolizy tanin | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Pohorecki, Ryszard