The importance of precultures for micropropagation of explants containing endophytes
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
An endophyte is a microorganism that spends most of its life cycle interor intra-cellularily of the host organism without causing its disease. As a resultof the high frequency of endophytes in plants, it is virtually impossible to isolate tissue or cell explants free from contaminants. Consequently, preculture(i.e. “stage 0”) is necessary before disinfecting and stabilisation of in vitro cultures, to eliminate explants that are sources of persistent contamination. Particularly dangerous are latent bacterial contaminants, which initially do not causeany symptoms and are propagated with plant material. Their presence becomesconspicuous after the passage to a sucrose-free medium or after transplantationto the soil.
Biotechnologia, vol.55, 4 (2001)-.
0860-7796 ; oai:rcin.org.pl:138591 ; IChB B-50
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 11, 2020
695
https://rcin.org.pl/ichb/publication/173925
Edition name | Date |
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Znaczenie prekultur w mikrorozmnażaniu roślin zasiedlonych przez endofity | Oct 2, 2020 |
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia
Broniarczyk, Justyna Koczorowska, Maria Magdalena Durzyńska, Julia Warowicka, Alicja Goździcka-Józefiak, Anna