Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, whichoriginated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LlNE-sequences. These were usedfor the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera. The IL-PCR technique using the above primers is describedand compared to reference methods such as Arbitrarily Primed-PCR and PulseField Gel Electrophoresis (PFGE).A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fmgerprints. A database for an automated yeast identification system is proposed.
Biotechnologia, vol.55, 4 (2001)-.
0860-7796 ; oai:rcin.org.pl:138601 ; IChB B-50
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
2 paź 2020
11 wrz 2020
56
https://rcin.org.pl/ichb/publication/173951
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Salek, Anna Teresa
Salek, Anna Teresa
Salek, Anna Teresa
Salek, Anna Teresa