Induction of genetic variation in vitro
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The effectiveness of traditional methods for inducing genetic variation hasgreatly increased with the introduction of various techniques in vitro. The newmethods of obtaining generative and somatic hybrids in vitro have resulted in agreater recombinant variation, exceeding the levels delimited hitherto by mating barriers. The potential for producing mutants has expanded due to the useof mutant somatic cells (brought in with the explant) as well as to the application of mutagens to individual cells and protoplasts, the haploid ones in particular. Two specific types of variation, i.e. somaclonal and gametoclonal variation,have proved to arise under the influence of various factors in tissue culture.However, the full application of these two types is inhibited to some extent bythe constrains on the regeneration ability of plants in culture, on the possibilityto select variants in vitro, and on the continuity of the resulting changes. Cultures in vitro also make it possible to introduce directional genetic changesthrough the application of molecular techniques.
Biotechnologia, vol.54, 3 (2001)-.
0860-7796 ; oai:rcin.org.pl:138883 ; IChB B-50
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 14, 2020
584
https://rcin.org.pl/ichb/publication/174176
Edition name | Date |
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Indukcja zmienności genetycznej in vitro | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Kruczkowska, Hanna Pawłowska, Helena Skucińska, Barbara
Kruczkowska, Hanna Pawłowska, Helena Skucińska, Barbara