Regeneration of Arabidopsis thaliana (L.) Heynh. plants via directsomatic embryogenesis
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
In Arabidopsis biotechnology plants are regenerated in vitro via shoot organogenesis induced in callus derived from different somatic tissues. An alternative way of in vitro plant regeneration via somatic embryogenesis has not beenapplied in Arabidopsis so far. Recently, it was found that development ofArabidopsis somatic embryos can be induced in the culture of immature zygotic embryos and that the callus phase is not nocessary for the initiation of embryogenesis. The aim of the presented research was to determinate the in vitro cultureconditions enabling high efficiency of somatic embryo induction and theirconversion into plants. The influence of induction medium composition including liquid or agar medium, type and concentration of auxin, carbohydrates andammonium sources as well as duration of auxin treatment of explants on DSEefficiency were evaluated. Advantages of described regeneration system via DSEare as follows: short time needed to induce somatic embryos (10-15 days), highefficiency ofthe process (more than 80% explants responded), numerous embryos produced per explant (on average 17) and high percentage of embryo conversion into fertile plants (70-80%).
Biotechnologia, vol.53, 2 (2001)-.
0860-7796 ; oai:rcin.org.pl:139269 ; IChB B-49
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 16, 2020
319
https://rcin.org.pl/ichb/publication/174627
Edition name | Date |
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Regeneracja roślin Arabidopsisthaliana (L.) Heynh. drogą bezpośredniej somatycznej embriogenezy | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Gaj, Małgorzata D. Czubin, Marta