Subtitle:

Entrapment of immobilised in situ intracellular lipase of Mucor in microporous capsules

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Lipases Mucor circinelloides and Mucor racemosus immobilised in situ were closed in microporous polysacharides hydrophilic gel cross-linked using a solutionof calcium salts. In order to increase the porosity of polysacharides matrix during its cross-linking oligomer molecules of ethylene oxide (optimal Polikol1000) were incorporated in the structure of the matrix. Then the oligomer wasremoved by acetone extraction. The obtained biocatalyst preparations were tested in hydrolysis of esters and esterification of oleic acid with butanol. The hydrolysis was carried out in water saturated organic solvents medium (n-hexaneand diisopropylether). It was found that the efficiency of M. racemosus lipase immobilisation in hydrolysis of n-butyl oleate, n-butyl palmitate, n-butyl stearate,n-butyl laurate amounted to 60%. The efficiency of M. circinelloides lipase immobilisation in esterification of oleic acid with 1-butanol in hexane achieved 45%

Relation:

Biotechnologia, vol.51, 4 (2000)-.

Volume:

51

Issue:

4

Start page:

142

End page:

151

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:140301 ; IChB B-47

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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