Subtitle:

In Vitro Culture of Cereal Microspores

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The development of microspore culture methods in the Poaceae family hasreceived considerable attention in recent years. Isolated microspore culture canbe induced in vitro to switch their development from gametophytic to a sporophytic patway, resulting in embryoid or callus formation. Different stresseslike cold or heat shock and nitrogen starvation have been identified as the major trigger in inducing microspore embryogenesis. Microspore culture appearsto be a promising tool for future production of double haploids in cereals. Isolated microspore culture has several advantages over anther culture in geneticmanipulation and haploid study, such as: direct observation of microspore development, unique possibility to study plant embryogenesis, easier in vitro selection and mutation, easier transformation of single cells. Moreover, isolatedmicrospores are the most efficient way of double haploid regeneration. Manyfactors such as genotypes, physiological status of donor plants, stage of microspores development, pretreatment of anthers or spikes, method of microsporesisolation, culture media, nurse culture and culture conditions, have a great influence on microspore culture. These and other problems concerning in vitroculture of isolated microspore are discussed in this review.

Relation:

Biotechnologia, vol.49, 2 (2000)-.

Volume:

49

Issue:

2

Start page:

142

End page:

160

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:141527 ; IChB B-45

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open