Inny tytuł:

Wykorzystanie linii komórkowych i metod synchronizacji cyklu komórkowego w klonowaniu ssaków

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Studies aimed at improving the effectiveness of cloning by nuclear transfer have shown thatproper development of the majority of reconstituted embryos is secured by G1 phase of thedonor nucleus or by using “universal recipients” i.e. enucleated pre-activated oocytes. Donornuclei for cloning may be derived from cell lines of embryonic stem cells (mouse), embryoniccells after short in vitro culture (sheep, pig, cattle) or even from fetal cells or adult cells afterin vitro culture and inducing quiescence in their nuclei (sheep).The use of fetal cells for transgenesis in vitro and the production of transgenic sheep afternuclear transfer from these cells opens the way to profitable technology of cloning transgenicfarm animals.

Czasopismo/Seria/cykl:

Biotechnologia, vol.41, 2 (1998)-.

Tom:

41

Zeszyt:

2

Strona pocz.:

86

Strona końc.:

100

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:144323 ; IChB B-37

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open