Inny tytuł:

Anti-HIV Activities and Mechanisms of Antisense Oligonucleotides

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Opis:

Polish - Japan Issue

Abstrakt:

We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides preventcDNA synthesis by the AMV, MMLV, and HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation ofthe RNA fragment bound to the antisense oligonucleotide by the reverse transcriptase associatedRNase H activity. However, the phosphorothioate oligomer inhibited polymerization by bindingto the AMV and MMLV RTs, rather than to the template RNA, whereas there was no competitivebinding of the phosphorothioate oligomer on the HIV RT during reverse transcription. Observation{of FlTC-S-ODN-reu-treated MOLT-4 cells with a confocal laser scanning microscope, revealedi diffuse fluorescence, apparently within the cytoplasm. Interestingly, fluorescent signals were; accumulated in the nuclear region of chronically infected MOLT-4/H1V-1 after a 60 min incubation. We also describe the long-term treatment of human immunodeficiency virus-infectedcells with antisense phosphorothioate oligonucleotides.

Czasopismo/Seria/cykl:

Biotechnologia, vol.35, 4 (1996)-.

Tom:

35

Zeszyt:

4

Strona pocz.:

116

Strona końc.:

131

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:146560 ; IChB B-31

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

eng

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open