Utility of starch raw materials for biosynthesis of glucose oxidase by Aspergillus niger
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Potato starch and its hydrolyzates were used to substitute glucose carbon sources to synthesize glucose oxidase (GOD) by free and immobilized on pumice stones A. niger mycelium.The basic conditions for starch hydrolysis, securing the maximal enzyme yields, were optimized.The immobilized A. niger mycelium was able to produce the enzyme at a small loss of its activity(about 28%) during ten 24-h batches. Free mycelium produced about 1.5 times higher GODactivity than the immobilized mycelium on glucose and starch hydrolyzates. However, the latterwas more stable, reaching faster the maximum of enzyme productivity, and it could be usedmany times with almost the same efficiency. Thus, the total enzyme yields produced by immobilized mycelium were much higher than those obtained using the free one.
Biotechnologia, vol.26, 3 (1994)-.
0860-7796 ; oai:rcin.org.pl:148481 ; IChB B-22
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
25 lis 2020
25 lis 2020
50
https://rcin.org.pl/ichb/publication/183891
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