Catalytical activity of penicillin acylase in native and stabilized derivatives
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Three different enzyme preparations were investigated: native enzyme, enzyme stabilized byadding poly(ethyleneimine) and enzyme attached to acrylic carrier. The preparations were compared with regard to their storage, temperature and pH-stabilities as well as the effect of temperature and pH-value on enzyme activity. It was found that for all preparations hydrolysis waskinetically limited. Five kinetic equation constants were calculated. The enzyme stabilized bypoly(ethyleneimine) was selected as the best preparation to be applied in industry.
Biotechnologia, vol.23, 4 (1993)-.
0860-7796 ; oai:rcin.org.pl:149104 ; IChB B-19
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
27 lis 2020
27 lis 2020
484
https://rcin.org.pl/ichb/publication/184607
Nazwa wydania | Data |
---|---|
Charakterystyka aktywności katalitycznej acylazy penicylinowej w formie wolnej i stabilizowanej | 27 lis 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Bryjak, Jolanta
Bryjak, Jolanta