Transgene expression and gene silencing in cereals
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Genetic transformation of cereal crops is a powerful research tool for analysis of gene function and varietal improvement. Application of the method is possible when the expression of introduced transgene is on the desired level and stable over several generations. The production of transgenic cereals was mainly performed by microprojectile bombardment. However, some advance was also achieved by application of Agrobacterium-med'iated transformation. For rice, which is the cereal model species, this method is routinely used, while for many others, especially polyploids, it has been developed very recently and only in a few laboratories. We still lack the knowledge whether the main features of Agro-mediated transformation (i.e. integration of one or few copies usually not rearranged and well defined transgene cassette) influence the transgene expression in polyploid cereal species. This review discusses known mechanisms possibly involved in transgene silencing, using both transformation methods. Part of the discussion is focused on transgene expression / silencing in relation to large genomes of polyploid cereals.
Biotechnologia, vol.75, 4 (2006)-.
0860-7796 ; oai:rcin.org.pl:87238 ; IChB B-71
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Dec 7, 2019
916
https://rcin.org.pl/ichb/publication/113743
Edition name | Date |
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Ekspresja transgenów i wyciszanie genów u zbóż | Oct 2, 2020 |
Nadolska-Orczyk, Anna
Nadolska-Orczyk, Anna Przetakiewicz, Anna Orczyk, Wacław
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan