Description:

Praca doktorska została wykonanaw Zakładzie Genomiki Strukturalnej RNA. Promotor:dr hab. Elżbieta Kierzek, prof. IChB PAN

Location:

Poznań

Abstract:

Every year influenza A virus causes 500 000 deaths worldwide. Influenza is importantresearch subject for scientists around the world because of its threat to humanity.The main goal of conducted research presented in this PhD dissertation wasdetermination of secondary structure of influenza A virus segment 5 vRNA (vRNA5). Theobject of the research was vRNA5 of strain A/Vietnam/1203/2004 (H5N1), which length is1565nt. Presented secondary structure of vRNA5 was predicted with constraints fromchemical mapping. Chemical mapping experiments were conducted with DMS, CMCT,kethoxal and NMIA. RNAstructure was used for secondary structure prediction usingexperimental data. Influenza A virus vRNA5 secondary structure was presented here for thefirst time. Secondary structure of vRNA5 has three domains: domain I (regions 1-69 nt and1285-1565 nt), domain II (70-797 nt) and domain III (798-1284 nt). In the domain III there ishighly structured region 1065-1281 nt with a stable hairpin 1074-1115 nt. Also, probability offorming base pairs in presented vRNA5 secondary structure was predicted.

Start page:

1

End page:

154

Detailed Resource Type:

PhD Dissertations

Format:

application/pdf

Resource Identifier:

oai:rcin.org.pl:204537

Language:

pol

Language of abstract:

eng

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

-

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open