Early Histochemical Changes in Perinatal Asphyxia

Mossakowski, Mirosław Jan (1929–2001); Long, D. M.; Myers, Ronald E.; Rodriguez de Curet, H.; Klatzo, Igor

Early Histochemical Changes in Perinatal Asphyxia

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Tytuł: Early Histochemical Changes in Perinatal Asphyxia

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Histochemical observations were carried out on newborn monkeys delivered by caesarean section at 153 to 163 days of gestation and immediately asphyxiated for approximately 12 minutes.The main histochemical finding consisted in widespread, abnormal accumulation of glycogen in glial cells (predominantly astrocytes) of both gray and white matter, which became conspicuous approximately 10 hours after asphyxia and tended to disappear after several days. Abnormal deposition of glycogen in glial cells was also demonstrated by electron microscopy.Glycogen accumulation was preceded by a marked increase in phosphorylases and UDPG-glycogen transferase activities already demonstrable in animals sacrificed 1 hour after asphyxia.Similarly early was the appearance of abnormal activity of aminopeptidase in neuronal groups which were shown to be especially susceptible to asphyxia and displayed evidence of histopathological damage at later stages.The changes in respiratory enzymes were generally of a nature previously described, and they were characterized mainly by reduction in the activity of individual enzymes in regions of the gray matter which later showed evidence of histopathological damage.Disturbances of the blood-brain barrier were demonstrable in animals sacrificed 10 hours and later after asphyxia. The main feature consisted in selective localization of the tracer in the cellular components of the parenchyma, mainly in the neurons. The changes were confined mostly to the gray matter without any clear correlation to the intensity of the histopathological damage.