Optimization of transduction of chosen cell lines using adenoviral vectors of the first generation
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene p-galactosidase (Adp-gal). We optimised the adsorption time and dose of Adp-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 lU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adp-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.
Biotechnologia, vol.78, 3 (2007)-.
oai:rcin.org.pl:78588 ; 0860-7796 ; IChB B-74
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Aug 16, 2019
756
https://rcin.org.pl/ichb/publication/102561
Edition name | Date |
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Optymalizacja warunków transdukcji wybranych linii komórkowych przy użyciu wektorów adenowirusowych pierwszej generacji | Oct 2, 2020 |
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja
Józkowicz, Alicja Dulak, Józef
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia