Enzymatic Hydrolysis of Waste Fats
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The intracellular lipases of Mucor circinelloides and Mucor racemosus immobilised in situ as well as the extracellular lipase of Rhizopus nigricans in solubleform were applied to enzymatic hydrolysis ofwaste fats from oil and meat industry, waste sludge of fats from municipal and industrial sewage-treatmentplants and waste water containing microemulsion of fats. The reaction of hydrolysis of plant and animal waste fats was carried out by two methods. Thefirst one was a partial hydrolysis of acylglycerols to obtain mono- and diacylglycerols. The second variant was complete hydrolysis of fats to obtain glyceroland free fatty acids. It has been demonstrated that lipase of Rhizopus nigricanspartly hydrolysed waste fats. The products of hydrolysis contained 35-45% mono- and diacylglycerols. These products may be used as emulsifier in the hydrolysis of fats. The yield of hydrolysis of waste fats by immobilised Mucor lipases was from 85 up to over 95%. Waste sludge of fats from municipal and industrial sewage contain 12-62% of free fatty acids. This kind of material was hydrolysed without an emulsifier but with the addition of calcium chloride. Theyield of hydrolysis of acylglycerols was from 45 to 76%. Tne yield of hydrolysis ofmicroemulsion of fats in waste water by immobilised Mucor lipases was from 80up to over 95%. As the hydrolysis products contained free fatty acids, mono- anddiacylglycerols, it was easy to make emulsions which could be utilised in anaerobic-aerobic processes.
Biotechnologia, vol.49, 2 (2000)-.
0860-7796 ; oai:rcin.org.pl:141525 ; IChB B-45
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 12, 2020
Oct 5, 2020
938
https://rcin.org.pl/ichb/publication/176828
Edition name | Date |
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Enzymatyczna hydroliza tłuszczów odpadowych | Oct 12, 2020 |
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