Stopa, Magdalena ; Jaźwa, Agnieszka ; Mleczko, Katarzyna ; Dulak, Józef ; Józkowicz, Alicja
Production of adenoviral vectors of the first generation - optimization of the method
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The paper presents the production of adenoviral vectors (AdV) containing P-galactosidase (Adp-gal), from the transfer of recombinant viral DNA into packing cell line (HEK293) to the titration of viral particles. Optimisation of the methods (preparation of DNA for transfection, adsorption time during the infection of cells, amount of serum in the medium, time-point of vector isolation) enables obtaining a titer of up to 10'° lU/mL. Adp-gal were titrated with several methods, with P-gal in situ staining used as a reference. We found that the most suitable titration method of the vectors containing other than reporter genes was the Rapid Titer ELISA kit™.
Biotechnologia, vol.78, 3 (2007)-.
0860-7796 ; oai:rcin.org.pl:76143 ; IChB B-74
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Jul 27, 2019
843
https://rcin.org.pl/ichb/publication/100059
Edition name | Date |
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Produkcja wektorów adenowirusowych pierwszej generacji - optymalizacja metody | Oct 2, 2020 |
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja
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