The most important features of adenoviral vectors
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
One of the major hurdles to successful gene therapy is the ability to efficiently introduce a foreign nucleic acid into the tissue of interest. As adenoviruses posses ability to enter rapidly a mammalian host cell and achieve propagation, they are widely used as a tool for delivery transgenes. Recombinant adenoviral vectors of first generation (AdV) contain an expression cassette with exogenous genes and are made replication deficient by the deletion of the E1/E3 region. They offer many advantages for gene delivery: ability to transduce a wide variety of cell types in a cell-cycle independent manner, easy and cheap propagation process to high titers and low pathogenicity for humans. However, AdV also have some disadvantages, namely cytotoxity, immunogenity, transient expression of transgene, which are mostly important in case of clinical trials. Despite those limitations and development of more sophisticated adenoviral systems (helper-dependent adenoviral vectors), AdV of first generation are still widely used for transducting different cell types in vitro, especially those that are refractory to other gene transfer methods, as well as in gene therapy clinical trials.
Biotechnologia, vol.78, 3 (2007)-.
0860-7796 ; oai:rcin.org.pl:75476 ; IChB B-74
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Oct 2, 2020
Jul 22, 2019
9025
https://rcin.org.pl/ichb/publication/99292
Edition name | Date |
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Najważniejsze cechy wektorów adenowirusowych | Oct 2, 2020 |
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