Search for: [Abstract = "Efficacy of adenoviral vectors \(AdV\) was checked by transduction of six different cell lines \(COS\-7, HUVEC, HMEC\-1, NIH 3T3, HaCaT, and B16\(F10\)\) with the vectors containing reporter gene p\-galactosidase \(Adp\-gal\). We optimised the adsorption time and dose of Adp\-gal. The transduction with efficacy of up to 100% was obtained for the doses 10\-100 lU\/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines \(HMEC\-1, HUVEC, HaCaT\), we found the induction of IL\-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adp\-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells."]

Number of results: 1