Subtitle:

Instability of ploidy level during regeneration of transformed andnon-transformed tobacco (Nicotiana tabacum L.)

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

In plant tissue cultures, somaclonal variation is often observed. It can be aneffect of the changes in the individual chromosome number or in the ploidylevel. Flow cytometry, a fast and accurate method for the estimation of the nuclear DNA content, can be applied to study these changes. The DNA content indifferentiated tissues of Nicotiana tabacum cultured in vitro was estimated usingPartec CCA flow cytometer, starting from explant, through callus, up to regenerated shoots. The explant constituted stem segments of N. tabacum plants,non-transformed and transformed with gfp gene. Flow cytometric analysisshowed differences in the proportion of 2C, 4C, 8C and 16C cells in plant tissuein different culture stages. Among the regenerated plantlets originated fromnon-transformed and transformed plants, diploid, tetraploid and mixoploidforms were observed. The transformation did not influence the share of cellsrepresenting different ploidy levels in the investigated plant material.

Relation:

Biotechnologia, vol.62, 3 (2003)-.

Volume:

62

Issue:

3

Start page:

260

End page:

266

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:133333 ; IChB B-58

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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