The aim of the study was an early detection of somaclonal variation (SV) which could occur within the micropropagated plant material. Shoot cultures of the Polish cultivars were used. Five cultivars derived from breeder A and four from breeder B. They were propagated in vitro for one or two years. The molecular markers (inter-simple sequence repeat, ISSR) were utilized for analysis of putative DNA polymorphism between standard plants (propagated traditionally) belonging to tested cultivars and somaclonal variants derived from them. Within the nine studied genotypes, for five of them, the ISSR analysis performed with ten primers did not reveal polymorphism between standard and micropropagated plants. The analysis of the other four cultivars, all derived from breeder B, showed that some of the plants, micropropagated either for one or two years, differed slightly from standard. Basing on ISSR data, the L'PGMA dendrogram showing genetic similarity of the analysed plants was generated and clusters grouping cultivars, their standards and micropropagated plants were noted.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Oct 2, 2020
May 27, 2019
|Ocena stabilności genetycznej rozmnażanych in vitro polskich odmian tulipanów przy użyciu markerów molekularnych ISSR||Oct 2, 2020|
Kuras, Anita Korbin, Małgorzata Żurawicz, Edward
Podwyszyńska. Małgorzata Gabryszewska, Eleonora Korbin, Małgorzata Jasiński, Artur
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.