Łuczak, Magdalena ; Figlerowicz, Marek ; Wojtaszek, Przemysław
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Proteomics is a scientific discipline that focuses on the large-scale study of proteins, particularly their structures, functions and interactions. The proteome is the full complement of proteins expressed by genome. During the last decade, thanks to subset of proteomic techniques and workflows, it has been possible to identify: diagnostic protein biomarkers, potential therapeutic targets and also biotechnologically important plant proteins, like those that give a plant resistance to drought. Over the last years, proteomics has generated a relatively large number of reviews on technical aspects and concepts. Although total automation and reproducibility are possible, the protocols of protein isolation and separation are sample specific. Moreover, most of them address the development of optimal sample preparation protocols of mammalian cells and tissue. In contrast, plant proteomics is still in its infancy, probably because plant material is very recalcitrant. The objective of this review is to pay attention to some methodological aspects of proteomic analyses using 2DE and mass spectrometry, especially in the case of plant material.
Biotechnologia, vol.85, 2 (2009)-.
0860-7796 ; oai:rcin.org.pl:72110
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Jun 3, 2019
3514
https://rcin.org.pl/publication/94507
Edition name | Date |
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Aspekty metodyczne analiz proteomicznych z wykorzystaniem metod elektroforezy dwukierunkowej i spektrometrii mas | Oct 2, 2020 |
Rodakowska, Ewelina Kasprowicz, Anna Łapa, Agnieszka Łuczak, Magdalena Derba, Marta Wojtaszek, Przemysław
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan