Isoenergetic microarrays, a new method to study the structure of RNA
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
RNA is one of the most important molecule essential for proper functioning of the cell. However, there are a growing number of RNAs for which biological function and structure are not known. Isoenergetic microarrays have been designed and apply as a new method to study the structure and interactions of RNAs. Isoenergetic microarrays are built with short modified RNA probes. The 2’-0-methylated and LNA-modified oligomer probes have been designed in such a way, that the interaction of each probe with its complementary single stranded fragment of target RNA has comparable thermodynamic stability (is isoenergetic). This method was tested and optimized on several native RNAs.
Biotechnologia, vol.83, 4 (2008)-.
0860-7796 ; IChB-78 ; oai:rcin.org.pl:73257
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
2 paź 2020
18 cze 2019
279
https://rcin.org.pl/publication/95945
Nazwa wydania | Data |
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Mikromacierze izoenergetyczne, nowa metoda badania struktury RNA | 2 paź 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.