Sposoby identyfikacji markerów RAPD w rośiinach
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
The random amplified polymorphic DNA (RAPD) method is based on random amplificationof DNA fragments, via PCR, using short primers of arbitrary sequence. RAPD markers have beenapplied to construct linkage maps, to assess genetic diversity, to study taxonomic relationships,and to tag disease resistance genes in plants. RAPD markers linked to a resistance gene canbe identified using bulked segregant analysis (BSA), recombinant inbred lines (RlLs) or nearisogenic lines (NlLs). More reliable and specific PCR-based markers known as sequence-characterized amplified region (SCAR) and allele-specific associated primer (ASAP) were developed. Thereare several examples of the application of these DNA marker systems in marker-assisted plantbreeding.
Biotechnologia, vol.44, 1 (1999)-.
0860-7796 ; oai:rcin.org.pl:143454 ; IChB B-40
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Nov 18, 2020
Oct 19, 2020
766
https://rcin.org.pl/publication/178646
Edition name | Date |
---|---|
Sposoby identyfikacji markerów RAPD w rośiinach | Nov 18, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.