Sposoby przechowywania materiału roślinnego w kulturach in vitro
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Tissue culture has been utilized for producing virus free plants for vegetative propagationand genetic engineering. In addition, altered cells lines showing higher productivity of secondarymetabolities can be obtained using tissue culture. The wide use of tissue culture requires thedevelopment of new preservation techniques for in vitro culture material.One of this method is growth reduction achieved by modifying various parameters such as:temperature, culture medium, gasous environment. However, crypreservation (i. e. storage inliquid nitrogen -196°C) is the only method available nowadays for long-term conservation. Newcryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccationhelped expend the list of plant species that can tolerate low temperatures and are characterizedby a normal rate of growth. Each step of the cryopreservation procces requires specific conditions
Biotechnologia, vol.40, 1 (1998)-.
0860-7796 ; oai:rcin.org.pl:144628 ; IChB B-36
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
18 lis 2020
27 paź 2020
928
https://rcin.org.pl/publication/180064
Nazwa wydania | Data |
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Sposoby przechowywania materiału roślinnego w kulturach in vitro | 18 lis 2020 |
Marecik, Roman Króliczak, Paweł Cyplik, Paweł
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Króliczak, Paweł Grajek, Włodzimierz Tomkowiak, Michał
Kamińska, Dorota Wesołowska, Maria Króliczak, Paweł Skrzypczak, Lutosława Grajek, Włodzimierz