Inny tytuł:

Resistance of transgenic tobacco lines to Potato Virus Y

Współtwórca:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Wydawca:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Abstrakt:

Potato virus Y (PVY) causes a disease which reduces the quality of raw material as well as the yield. This study on resistance included tobacco transgenic lines belonging to four cultivars modified with three genetic constructions, containing Lettuce mosaic virus coat protein gene (LMV CP) and PVY replicase gene used in sense (ROKY1) and antisense (ROKY2) orientation. The plants were inoculated with PVY isolates under greenhouse conditions and tested by DAS-ELISA. The highest effectiveness of antiviral protection was noticed in case of the lines with transgene LMV CP, lower for ROKY2, and the lowest for ROKY1. In following generations, the percentage increase of a number of plants resistant to PVY was observed within the lines containing transgene LMV CP. Cultivar MN 944 showed to be the best genetic background for that transgene, while cultivar AC Gayed for transgene ROKY.

Czasopismo/Seria/cykl:

Biotechnologia, vol.89, 2 (2010)-.

Tom:

89

Zeszyt:

2

Strona pocz.:

72

Strona końc.:

82

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

oai:rcin.org.pl:71582 ; IChB B-84

Źródło:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open