Handschuh, Luiza ; Lewandowski, Krzysztof ; Kaźmierczak, Maciej ; Komarnicki, Mieczysław ; Figlerowicz, Marek
Blood proliferative diseases - application of the DNA microarrays for transcriptome analysis
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Hematopoiesis is a complex process precisely regulated by a wide spectrum of cooperating factors. Dysfunction of hematopoietic cell proliferation, differentiation or maturation usually leads to the malignant transformation. The DNA microarray-based transcriptome analysis helped to revise the traditional classification of hematological disorders, predict their outcome, test potential therapeutic agents and better understand basic mechanisms underlying cancer origin and development. Here, the results of gene expression profiling in myelo- and lymphoproliferative diseases such as leukemia, lymphoma and myelodysplastic syndromes, are presented. Two microarray technologies were applied in this area of research: Aflfymetrix gene chips and cDNA microarrays. Among them, Lymphochip is a prominent example of a specialized cDNA microarray tool designed to investigate gene expression in the immunological system and hematological diseases. It seems that typical problems connected with microarray results analysis - small number of patients, loss of reproducibility can be overcome by increasing the number of samples and application of identical protocols, equipment and reagents in different laboratories.
2009 ; Biotechnologia, vol.86, 3 (2009)-.
0860-7796 ; oai:rcin.org.pl:71950 ; IChB B-81
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
2 paź 2020
30 maj 2019
468
https://rcin.org.pl/publication/94371
Nazwa wydania | Data |
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Choroby rozrostowe krwi - analiza transkryptomu z zastosowaniem mikromacierzy DNA | 2 paź 2020 |
Handschuh, Luiza Magacz, Tomasz Figlerowicz, Marek
Formanowicz, Piotr Urbaniak, Radosław Handschuh, Luiza Formanowicz, Dorota Figlerowicz, Marek
Żmieńko, Agnieszka Handschuh, Luiza Góralski, Michał Figlerowicz, Marek
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan