Object

Title: Cryopreservation as a method to facilitate presenation of life capacity of plant cells and tissues

Creator:

Mikuła, Anna

Date issued/created:

2008

Resource Type:

Article

Subtitle:

Cryopreservation as a method to facilitate presenation of life capacity of plant cells and tissues

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultra- structural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.

Relation:

Biotechnologia, vol.81, 2 (2008)-.

Volume:

81

Issue:

2

Start page:

41

End page:

57

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:73837 ; IChB B-76

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Object collections:

Last modified:

Sep 19, 2019

In our library since:

Jun 26, 2019

Number of object content hits:

6

All available object's versions:

https://rcin.org.pl/publication/96631

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