This paper aims to evaluate different forms of the enzyme-linkedimmunosorbent assays (ELISA) for the detection of virus-specific antibodies andfocuses on factors which may influence the diagnostic reliability of such tests.The differences between competitive and non-competitive ELISAs are described, with reference to the influence of the antigen, the conjugated antibody, and the test sample on the test results. Attention is drawn to interference, which may result in false positive or false negative test results. The use ofmonoclonal antibodies and discriminatory tests is briefly discussed. Diagnosticreliability is described for tests that are used in monitoring or eradicationprogrammes, emphasising the consequences of false negative or false positiveresults.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Oct 2, 2020
Sep 14, 2020
|Enzymatyczny odczyn immunoadsorpcyjny (ELISA) jako metoda detekcji specyficznych przeciwciał w surowicach zwierząt||Oct 2, 2020|
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Szewczyk, Bogusław Bieńkowska-Szewczyk, Krystyna
Nowak, Jacek K.
Kochan, Grażyna Szewczyk, Bogusław