The aim ofthe performed investigations was to determine which part oftheembryo has the optimal properties for callus induction at low concentrations of2,4-D (2 mg dm-3).The explants were cut along or across the embryo axis into 2, 3, 4 fragments and cultured on the Murashige and Skoog medium for 6 weeks. It wasfound that callus derived from mature embryos (dry seeds) is characterised bycomparable fresh and dry mass and by similar changes in size of the cells withfragments derived from mature embryos (but before the dormant) and from immature embryos. Essential difference was observed in the germination ability ofthe explants. The percentage of germination of mature embryo fragments wasthe lowest. It was demonstrated that despite of the embryo type, fragmentsoriginating from its central part appeared the most suitable for in vitro culture.In the case of these fragments the highest increase of the fresh mass of callustissue, optimal morphological features and the appearance of meristematic centres were observed.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Oct 2, 2020
Sep 17, 2020
|Możliwości wykorzystania różnych fragmentów zarodka kukurydzy (Zea mays L.) w hodowli in vitro||Oct 2, 2020|
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.
Marcińska, Izabela Filek, Maria Biesaga-Kościelniak, Jolanta Pilipowicz, Marta
Zimny, J. Czaplicki, A. Menke-Milczarek, I. Oleszczuk, S. Sowa, S.