Inny tytuł:

Germin genes expression induced by stress factors in transgenic tobacco plants (Nicotiana tabacum var. Xanthi)

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Wheat germin, a glycoprotein mainly localized in the cell wall of monocots,dicots and pine [Pinus caribaea Morelet), is a specific marker of the onset ofgrowth in germinating seeds. There are two genes gf-2.8 and gf-3.8 of germin,isolated from wheat (Triticum aestivum). Because of their structure and biochemistry, both of the germin’s isoforms have been now assimilated to the oxalate-oxidase enzyme (E.C. 1.2.3.4). Several constructs of the gf-2.8 and gf-3.8genes promoters deletion were fused with uidA gene and tested for the GUS activity in the transgenic tobacco plants. The aim of this work was to evaluatewhether some environmental factors induce the activity of germin. Hormonaltreatment (auxins 2,4-D, NAA, giPberellin GA3), salt treatment, salicylic acid,heavy metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Hg, As) and A1 induced high GUS activity in root apex, cotyledons and hypocotyls in transgenic seedlings and in leavesof the tobacco plants transformed with the entire and one partially deleted construct of the gf-2.8 gene. Neither cold nor heat shock, enhanced significant GUSactivity. The gf-3.8 - GUS constructs of germin did not give a positive responseto any factors mentioned above. Thus, only the gf-2.8 protein seems to be regulated by some factors. The gf-2.8 oxalate-oxidase activity could be then involvedin general stress-induced signalling in higher plants.

Czasopismo/Seria/cykl:

Biotechnologia, vol.53, 2 (2001)-.

Tom:

53

Zeszyt:

2

Strona pocz.:

152

Strona końc.:

164

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:139324 ; IChB B-49

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open