Subtitle:

Antisense oligonucleotides as a tool to study gene expression in the brain

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Antisense oligonucleotides (asODN) have recently been used to block specific gene expressionin the rodent brain. Their targets include subunits of receptors for neurotransmitters, neuropeptides and transcription factors, i.e. those proteins, whose other blockers are not known.Successful applications of the asODN require good understanding of their pharmacokinetics,mechanisms of action and side effects in the brain. Unfortunately, very little is known in thisregard. Both intraventricular and intrastructure route of administration of phosphorodiester (O- ODN) and phosphorothioate (S - ODN) ODN to the brain were effectively employed. However,doses used, even in the case of the same analog, differ up to two orders of magnitude. Sincetranslation arrest is believed to be an effective mechanism of ODN activity in the brain, mostof the authors target the ODN to the mRNA region including the translation codon, but thereare almost no studies of the target mRNA levels. The paper reviews the recent development inthis field, offering critical evaluation of the data.

Relation:

Biotechnologia, vol.28, 1 (1995)-.

Volume:

28

Issue:

1

Start page:

65

End page:

73

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:148049 ; IChB B-24

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open