Enzymatic synthesis of esthers in organic solvents. Selected problems
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Water, which is the natural environment of vital processes, is essential for lipases activity in vivo, but invitro it can be substituted by an organic solvent. Under these novel conditions lipases exhibit the ability ofesther synthesis. The replacement of water with an organic solvent is qualitatively and quantitatively limited because of chemical and physical properties of organic solvents. The qualitative limitations are theconsequence of the destruction of catalytic conformation (as a resulting from) polar reactions. The quantitative limitations are also the result of polar reactions which are indirectly expressed as the ability of an organic solvent to mix with water and are realised as the penetration of environmental ingredients to the essential water layer which covers the enzyme molecules. Both the limit and the kind of the penetrationdirectly affect the preservation of the enzyme catalytic conformation.The indispensable water layer which thermodynamically stabilizes the enzyme molecule in the environment of organic solvent is partially replaced by other environmental ingredients which form hydrogenbonds with functional and active groups of protein i.e. formamide, DMF, pyridine, that in the final consequence may cause an increase of en::> latic activity. In this paper the examples of lipases applications forsynthesis of various esthers are deser- ed.
Biotechnologia, vol.11, 1 (1991)-.
0860-7796 ; oai:rcin.org.pl:181769 ; IChB B-08
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Apr 13, 2021
Apr 6, 2021
305
https://rcin.org.pl/publication/216132
Edition name | Date |
---|---|
Enzymatyczna synteza estrów w środowisku rozpuszczalników organicznych. Wybrane zagadnienia | Apr 13, 2021 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.
Antczak, Tadeusz Szczęsna-Antczak, Mirosława
Antczak, Tadeusz Szczęsna-Antczak, Mirosława
Antczak, Tadeusz Galas, Edward