The genetic fidelity of the micropropagated daylily plants was evaluated by a phenotypic observation and with the molecular markers, randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques. The plants of nine daylily cultivars propagated in vitro for the period of one to five years and grown outdoors in the years 2002 to 2007 were used in the study. The somaclonal variants occurred within the micropropagated plants of the five genotypes. The somaclones differed from the true-to-type plants in terms of colour and shape of the flowers. Depending on the genotype, the frequency of somaclonal variation (SV) ranged from 1.4% to 100%. The RAPD and ISSR analyses of the selected somaclones confirmed the polymorphism between true-to-type plants and somaclones on the level of DNA.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Oct 2, 2020
May 27, 2019
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Wojtania, Agnieszka Gabryszewska, Eleonora