Inny tytuł:

Somaclonal variation in micropropagated Hemerocallis sp. plants determined by phenotype and molecular markers, RAPD and ISSR

Współtwórca:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Wydawca:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Abstrakt:

The genetic fidelity of the micropropagated daylily plants was evaluated by a phenotypic observation and with the molecular markers, randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques. The plants of nine daylily cultivars propagated in vitro for the period of one to five years and grown outdoors in the years 2002 to 2007 were used in the study. The somaclonal variants occurred within the micropropagated plants of the five genotypes. The somaclones differed from the true-to-type plants in terms of colour and shape of the flowers. Depending on the genotype, the frequency of somaclonal variation (SV) ranged from 1.4% to 100%. The RAPD and ISSR analyses of the selected somaclones confirmed the polymorphism between true-to-type plants and somaclones on the level of DNA.

Czasopismo/Seria/cykl:

Biotechnologia, vol.89, 2 (2010)-.

Tom:

89

Zeszyt:

2

Strona pocz.:

114

Strona końc.:

124

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

oai:rcin.org.pl:71605 ; IChB B-84

Źródło:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open