Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The study purpose was to examine the influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique. Two methods of DNA isolation were used - the Tomson-Henry method, which provides low purified DNA, and the column method, which provides high purified DNA. By using DNA isolated with these two methods the RAPD analysis of genetic distance was executed. It was noticed, that using high purified DNA resulted in higher number of amplification’s products, and consequently in larger accuracy of analysis.
Biotechnologia, vol.86, 3 (2009)-.
0860-7796 ; oai:rcin.org.pl:72000 ; IChB B-81
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
2 paź 2020
31 maj 2019
2460
https://rcin.org.pl/publication/94420
Nazwa wydania | Data |
---|---|
Wpływ metody izolacji DNA na wynik analizy podobieństwa genetycznego wykonanej techniką RAPD | 2 paź 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Turczynowska, Anna Broda, Zbigniew
Broda, Zbigniew Mikołajczyk, Sylwia