Inny tytuł:

The influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

The study purpose was to examine the influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique. Two methods of DNA isolation were used - the Tomson-Henry method, which provides low purified DNA, and the column method, which provides high purified DNA. By using DNA isolated with these two methods the RAPD analysis of genetic distance was executed. It was noticed, that using high purified DNA resulted in higher number of amplification’s products, and consequently in larger accuracy of analysis.

Czasopismo/Seria/cykl:

Biotechnologia, vol.86, 3 (2009)-.

Tom:

86

Zeszyt:

3

Strona pocz.:

182

Strona końc.:

191

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:72000 ; IChB B-81

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

2009

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open