Suspension cultures of Pharbitis nil
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Suspension cultures are more suitable for physiological, biochemical andmolecular investigations than callus cultures grown on solid media, because theformer provide more homogeneous system than the latter ones. A large numberof plants were found suitable for establishing cell suspension cultures. In thisstudy we describe the obtaining of cell suspension cultures derived from callusinduced from cotyledons of Pharbitis nil. Explants isolated from plants grownunder inductive and non-inductive conditions were cultured on MS basal medium containing various concentrations and combinations ofgrowth regulators.To initiate the cell suspension culture, small clumps of friable callus obtainedfrom cotyledons were suspended in liquid callusing medium. An initial inoculumdensity was 2 x lO'^cells/ml. Every 20 days the cultures were transferred to afresh medium. The cell number in suspension was determined by direct microscopic counting with haemocytometr. The cell suspension cultures containedboth single cells and small cell aggregates
Biotechnologia, vol.52, 1 (2001)-.
0860-7796 ; oai:rcin.org.pl:140015 ; IChB B-47
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
2 paź 2020
19 wrz 2020
59
https://rcin.org.pl/publication/175471
Nazwa wydania | Data |
---|---|
Uzyskiwanie zawiesin komórkowych Pharbitis nil | 2 paź 2020 |
Szyp, Iwona Dąbrowska, Grażyna Tretyn, Andrzej
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Szyp-Borowska, Iwona Staniulyte, Ramune
Trejgell, Alina Tretyn, Andrzej