Inny tytuł:

B-galactosidase activity of proteins extracted from Sulfolobus shibatae

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

The cytoplasmic p-galactosidase/p-glucosidase from hyperthermophilicarchaeon Sulfolobus shibatae has been characterized with regard to its use in lactose hydrolysis. Cell extract was purified 16-fold to a specific activity 29.5 U/mgusing ammonium sulfate precipitation, ion-exchange chromatography, and gelfiltration. Isolated enzyme exhibited optimum activity at pH 5.5 and 98°C andhad a half-life of 7 h in acetate buffer (pH 5.5) at 90°C. Cu2+, Hg2+ and Zn^+strongly inhibited the enzyme, whereas catalytic properties of other investigated cations were barely influenced. Glucose and galactose were predominantly produced from lactose. However, at the substrate concentration of 0.15 M,small amount of lactose was converted into transgalactosylation products.

Czasopismo/Seria/cykl:

Biotechnologia, vol.61, 2 (2003)-.

Tom:

61

Zeszyt:

2

Strona pocz.:

268

Strona końc.:

279

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:135754 ; IChB B-57

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open